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| Gava,Fábio Nelson; Silva,Sheila Nogueira Saraiva da; Rosa,Fernando Azadinho; Ortiz,Edna Mireya Gómez; Rodrigues,Bruno Cristian; Bandarra,Márcio de Barros; Vasconcelos,Rosemeri de Oliveira; Camacho,Aparecido Antonio. |
| ABSTRACT: Cardiotoxicity induced by doroxubicin generates systolic disfunction and myocardial remodeling with presence of myofibroblasts. These cells are thought to be attracted to the injured heart to avoid the development of congestive heart failure. The objective of this study was to evaluate the systolic dysfunction generated by doxorubicin through Doppler echocardiography, and its correlation with the presence of myofibroblasts in the myocardium. Twenty-five New Zealand White rabbits were divided into two groups (control, and treated with doxorubicin). The drug was administered for six weeks; Doppler echocardiography was performed before the first, and after the last administration of doxorubicin. Immuno detection of myofibroblasts was performed by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Α-SMA; Doxorubicin; Doppler echocardiography; Rabbits. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901642 |
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| Wang,Q.; Wang,L.X.; Zeng,J.P.; Liu,X.J.; Liang,X.M.; Zhou,Y.B.. |
| Liver cirrhosis is one of the most common diseases of Chinese patients. Herein, we report the high expression of a newly identified histone 3 lysine 4 demethylase, retinoblastoma binding protein 2 (RBP2), and its role in liver cirrhosis in humans. The siRNA knockdown of RBP2 expression in hepatic stellate cells (HSCs) reduced levels of α-smooth muscle actin (α-SMA) and vimentin and decreased the proliferation of HSCs; and overexpression of RBP2 increased α-SMA and vimentin levels. Treatment with transforming growth factor β (TGF-β) upregulated the expression of RBP2, α-SMA, and vimentin, and the siRNA knockdown of RBP2 expression attenuated TGF-β-mediated upregulation of α-SMA and vimentin expression and HSC proliferation. Furthermore, RBP2 was highly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RBP2; Liver cirrhosis; Fibrosis; Α-SMA; Vimentin; HSC. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900739 |
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| Santos, Jomel Francisco dos; Franco, Matheus Castro; Lima, Ueliton Assis de; Maciel, Thiago Arcoverde; Bandarra, Márcio de Barros; Oliveira, Daniela. |
| O presente trabalho empregou técnicas de imuno-histoquímica e citoquímica para avaliar a presença de componentes-chave para o desenvolvimento da contratura articular na espécie ovina e a potencialidade desta espécie como modelo experimental para estudo desta patologia. Para tanto, foram utilizadas 15 cápsulas articulares de joelhos de ovelhas Santa Inês sadias para localização de miofibroblastos e mastócitos. Para investigação de miofibroblastos foi realizada a técnica de imuno-histoquímica. Após a preparação para rotina histológica, a recuperação antigênica foi realizada por aquecimento em citrato, seguida pelo bloqueio das peroxidades, bloqueio de proteínas inespecíficas e então, o anticorpo primário foi incubado. Após, o anticorpo secundário foi... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cápsula articular; Eixo fibrose; Ovelhas; Α-SMA; Mastócitos. |
| Ano: 2015 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/25261 |
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